10/6/2023 0 Comments Download NivalisAggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol. Spin the vial gently until full dissolution. The lectin may be reconstituted with 2 ml of deionized water before use. Model systems to help understand the molecular basis of how proteins recognize carbohydrates.The lectin is available in vials containing 5 mg powder and is to be used for laboratory work only. The purity of GNA lectin is determined by SDS- electrophoresis, which generates one single band at 13 kDa corresponding to the four identical polypeptide chains. Medicago’s GNA lectin is supplied as a white to cream coloured lyophilized powder. It is also applicable for blood cell agglutination studies but also as a model system to help understand the molecular basis of how proteins recognize carbohydrates. Unlike the majority of mannose-binding lectins, GNA does not bind alpha-linked glucose. It contains little or no carbohydrate and does not need Ca 2+ or Mn 2+ for binding, since unlike most mannose-specific lectins it is not a metalloprotein. Structures containing (α-1,3) mannose residues are preferred for binding (2). It binds specifically to murine IgM immunoglobulin and human α 2-macroglobulin. The lectin is known to agglutinate rabbit erythrocytes but not human erythrocytes (1). Is has a molecular weight of 50 kDa and consists of four identical subunits (1). Galanthus nivalis lectin or agglutinin (GNA) is isolated from snowdrop bulbs. Binds to serum IgM and α 2- macroglobulin.Sugar specificity: ( α -1,3) mannose residues.*** For pack size 1 g or more, please contact us to inquire about pricing
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